We can look inside cells with great accuracy thanks to this innovative method

Today, thanks Innovations in microscopyIt is now possible to view the interiors of cells and other nanostructures. This is particularly the case for expansion microscopy or ExM. This innovative method Ultra-resolution imaging It makes it possible to obtain unprecedented images of the inside of our cells. It is based on physical zoom rather than optical zoom.

Thanks to this new microscopy technique, scientists can reach the infinite size. The appearance of this new method in microscopy will make it possible to implement it Great progress in neuroscience, pathology, and other biological or medical fields. today is Mr. Zhao’s Laboratory of BioImages Became a pioneer in the field of super-resolution imaging of biological samples using expansion microscopy.

During this revolutionary process, the samples are combined into a file Inflatable water gel which expand homogeneously to increase the distance between the particles. After this physical stretching, it is then possible to observe the tissues with higher accuracy.

Expansion microscopy allows images to be seen in high resolution

microscopic expansion More efficient One of today’s high-resolution imaging technologies, which is often very expensive. But the researchers were even able to visualize the biological structures in nanometer scale with this new technology.

In an article entitled “Magnify is a Universal Molecular Fixation Strategy for Expansion Microscopy”Researchers from Carnegie Mellon University, the University of Pittsburgh, and Brown University describe a new protocol they call Zoom in.

Zoom is Variant of dilation microscopy which uses a new hydrogel formula. This hydrogel invented by Professor Gao’s team is more efficient than its predecessors. retains a broader spectrum of biomolecules, Offers a wider application for a variety of fabrics and increases the stretch rate up to 11 times linearly, or approximately 1300 times the original size.

Magnify enables tissue monitoring without removing biomolecules

Zoom can become Powerful and accessible tool to the community Biotechnology. enabled them to overcome longstanding challenges with microscopic expansion. Magnify particularly allows the preservation of vital molecules within tissues such as proteins, nucleus fragments or carbohydrates. Reality Keeping the various biological components intact Within the expanded specimens is the first of its kind in microscopy.

Most operations require current Eliminate many biomolecules which may contain valuable research information. Previous protocols in expansion microscopy required the use of enzymes that facilitated cell expansion, but also digested proteins. At the end of the process, the scientists obtained images that show Empty slots for missing particles. Thanks to Magnify, they can now monitor proteins, fats, and carbohydrates simultaneously.

Source : scitechdaily